| Caroline Esther Nason Howard McWilliams | 1895-1898 |
| Cora Amelia Gray Brill | 1898-1900 |
| Minnie "Belle" Crouch Knuppe | 1900-1902, 1911-1912 |
| Mary J. King Johnston | 1902-1904 |
| Kate Hills Elliott | 1904-1905 |
| Joella Elsie Whitney Joy | 1905-1906 |
| Alice Adelbert Ames Hall | 1906-1908 |
| Ellen "Nellie" Coburn Jefferson | 1908-1910, 1912-1914, 1914-1916 |
| Alice Candace McCray Walther | 1910-1911 |
| Louise Dyer Griggs Johnston | 1916-1918, 1927-1929 |
| Blanche M. Houghton Forbes | 1918-1920 |
| Helen M. Griggs Barnes | 1920-1920 |
| Mary Elizabeth Prescott Jameson | 1920-1923 |
| Nannie-Bell Westover Skinner | 1923-1924 |
| Katherine M. Ryan Haman | 1924-1926, 1929-1930 |
| Delia Katherine Dukes Diether | 1926-1927 |
| Katherine Melrose Weber Ekstrand | 1930-1932, 1932-1934, 1940-1942, 1952-1954 |
| Lucia Winter Powell Stewart | 1934-1936 |
| Olive E. Whiting Stone | 1936-1937 |
| Marcie A. Alexander Orr | 1938 |
| Helen Alta Olds Stearns | 1938-1940 |
| Louise Emily Ward Clifford | 1942-1944 |
| Mabelle Barnes Metcalfe | 1944-1946, 1962-1964 |
| Helen Morris Lee Denison | 1946-1948 |
| Beulah Ruth Irwin Broady | 1948-1950 |
| Estella Nancy Miller Frank | 1950-1952, 1967-1969 |
| Elisabeth Anne "Betty" Broady Misuré | 1954-1956 |
| Ruby Jeane Offrell Kansas | 1956-1958 |
| Dorothy Greenstreet Anderson | 1958-1960 |
| Gevene Shirk McCreight | 1960-1962, 1965-1967 |
| Edith Burt Bigelow | 1964-1965, 1969-1972 |
| Margaret Hazel Crom Arnold | 1972-1975, 1979-1981, 1987-1989, 1991-1993, 1997-1999 |
| Joyce Bell Olson | 1975-1977 |
| Ellen Bowlin Boulware | 1977-1979 |
| Mary Margaret Tibbs Molina | 1981-1983 |
| Harriet Elizabeth Ventress Blilie | 1983-1985, 1985-1987, 1993-1995, 1995-1997, 1999-2001, 2001-2003, 2003-2005 |
| Margery Elaine Blake Rehm | 1989-1991 |
| Geraldine "Gerry" Rose Dellwo Larson | 2005-2007 |
| Debora Ann Blakeway | 2007-2009 |
| Marlene Ann King Johnson | 2009-2011, 2011-2013, 2013-2015 |
| Jennifer Lynn Parker | 2015-2017, 2017-2019 |
| Karrie Louise-Norenberg Blees | 2019-2021, 2021-2023 |
| Nancy Jane Olson Adomeit | 2024-2025 |
Also State Regent of The Orient: Caroline E. Nason McWilliams Holt
Also Minnesota State Regents: Mary Jameson, Laura Brown Duxbury
Mother-Daughter Regents: Beulah Broady & Betty Misuré
Longest-serving Regent: Harriet Blilie (7 two-year terms)
